Question
The redesign of this system is the goal of a project that developed the SCRaMbLE technique and announced the creation of a strain with a more than 50-percent synthetic version of this system in November 2023. For 10 points each:
[10h] Name or describe this eukaryotic system that will have its transposons, subtelomere regions, and introns removed, among other beneficial modifications, by its namesake 2.0 Project.
ANSWER: yeast genome [accept Saccharomyces cerevisiae genome; or S. cerevisiae genome; prompt on Sc2.0 or yeast 2.0; prompt on the genome, DNA, or chromosomes by asking “of what organism?”]
[10m] The SCRaMbLE system used to modify the yeast genome was developed to use the Cre (“cree”) recombinase, which recognizes these sites. These 34-base-pair recombination sites flank a gene of interest to be excised in a common knockout system.
ANSWER: LoxP [accept locus of x-over, P1; accept more specific answers like LoxPsym that include “loxP”; prompt on Cre-Lox]
[10e] The altered chromosomes in the modified yeast 2.0 genome have all of these sequences changed from TAG to TAA. These sequences mark the [emphasize] end of an open reading frame.
ANSWER: stop codons [prompt on codons]
<RH, Biology>
Summary
| 2024 Chicago Open | 07/28/2024 | Y | 15 | 11.33 | 87% | 27% | 0% |