A two-parameter mass action kinetic model is used in the MAKERGAUL software to analyze the results of the technique. Udvardi, Czechowski, and Scheible published “Eleven Golden Rules” of this technique that include using a Agilent 2100 Bioanalyzer to check quality. After a 2002 paper claimed to detect measles virus in children with autism using this technique, Stephen Bustin developed the MIQE (15[1])guidelines for performing this technique. The (*) Livak method and delta-delta-c method both normalize the cycle threshold reported by this technique. Hydrolysis probes like TaqMan are used to increase the specificity of this technique. It’s not gel electrophoresis, but this technique uses a fluorometer along with a Fluorescent dye like SYBR Green. The results of this technique are plotted on a melting curve. For 10 points, name this technique that monitors the amplification of DNA as it occurs. ■END■