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Robert Koch developed his four postulates to describe the causes of this disease. For 10 points each:
[10m] Name this disease. Koch also discovered PPD, a solution of proteins injected under the skin to screen for a wheal response to this disease in the Mantoux test.
ANSWER: tuberculosis [or TB; accept consumption]
[10h] Ziehl and Neelsen built on Koch’s work with the acid-fast stain, which stains bacteria such as M. tuberculosis with carbolfuchsin (“car-bohl-F’YOOK-sin”) and uses this dye as a counterstain. This dye is also used to count live eukaryotic cells, but is less useful for that purpose compared to a “trypan” dye of the same color.
ANSWER: methylene blue [or MB; or methylthioninium chloride; reject “methylene” or “methyl blue”] (Trypan blue only stains dead cells, while methylene blue can also stain live cells.)
[10e] Koch also developed these ubiquitous microbiology devices, which he used to discover M. tuberculosis. Fanny Hesse first proposed using agar gel in these shallow plastic plates.
ANSWER: Petri dishes
<AS, Biology>

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