This technique’s results are generally considered acceptable quality if rated twenty or higher on the Phred quality score. Characteristic squiggles are produced by samples passing through alpha-hemolysin connected to electrodes in a type of this technique developed by Oxford Nanopore. Bridge amplification is performed after adapters are ligated onto the ends of samples in this technique’s library preparation step so they can adhere to a flow cell. Coverage and depth are considered when designing experiments with this technique, which has platforms designed by Illumina for its “next-generation” form. Target molecules incorporate fluorescent ddNTPs as “chain-terminators” in a type of this technique developed by Sanger. For 10 points, name this technique that determines the order of nucleotides in DNA. ■END■
ANSWER: DNA sequencing [or RNA sequencing or DNA-seq or RNA-seq; accept Sanger sequencing or whole genome sequencing or next-generation sequencing or long-read sequencing or Oxford Nanopore sequencing or Illumina sequencing]
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