genetic competence - 2024 ACF Regionals @ Cornell

Question

Bacteria with the natural form of this property can undergo horizontal gene transfer with lysed cells. For 10 points each:

[10m] Name this property of bacterial cells that allows them to take up genetic material from their environment. Cells with this property can take up plasmids following electroporation or heat shock transformations.

ANSWER: genetic competence [or word forms like competent cells/bacteria; accept late competence]

[10h] The competence of a bacterial strain can be assessed by transforming it with this plasmid, named for its discovery at the University of California. This plasmid encodes an ampicillin resistance marker and a biologically active beta-galactosidase fragment.

ANSWER: pUC19

[10e] pUC19 is optimized to transform the TOP10 and DH10-beta strains of this gram-negative gut microbe. This model bacterium is by far the most common bacterial species used to transform plasmids in the lab.

ANSWER: E. coli [or Escherichia coli]

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Summary


2024 ACF Regionals @ Berkeley	01/27/2024	Y	3	13.33	100%	33%	0%
2024 ACF Regionals @ Cornell	01/27/2024	Y	3	10.00	100%	0%	0%
2024 ACF Regionals @ JMU	01/27/2024	Y	10	13.00	100%	20%	10%
2024 ACF Regionals @ Minnesota	01/27/2024	Y	2	10.00	100%	0%	0%
2024 ACF Regionals @ Nebraska	01/27/2024	Y	6	16.67	100%	33%	33%
2024 ACF Regionals @ Ohio State	01/27/2024	Y	2	10.00	100%	0%	0%
2024 ACF Regionals @ Rutgers	01/27/2024	Y	4	17.50	100%	50%	25%
2024 ACF Regionals @ Vanderbilt	01/27/2024	Y	5	10.00	100%	0%	0%

Data


Cornell B	Binghamton	10	10
Cornell A	RIT A	10	10
RIT B	Cornell C	10	10