Question
Kary Mullis was inspired to develop this technique after LSD-induced visions of a fluorescent raccoon. For 10 points each:
[10e] Name this technique which cycles between denaturation, annealing, and extension to amplify segments of DNA. Its quantitative form is commonly used to quantify gene expression.
ANSWER: PCR [or polymerase chain reaction]
[10m] Like PCR, this technique can be used to detect the presence of a specific sequence or gene in a specimen. This technique uses a labeled, often fluorescent DNA or RNA probe and identifies where it binds, typically within a chromosome.
ANSWER: in situ hybridization [accept FISH]
[10h] This technique for finding protein-DNA interactions crosslinks DNA and proteins, shears the specimen, then uses antibodies to pull the fragments before analysis.
ANSWER: chromatin immunoprecipitation [or ChIP]
<AG, Biology>
Summary
| 2023 ILLIAC (Cornell) | 2023-10-21 | Y | 1 | 0.00 | 0% | 0% | 0% |
| 2023 ILLIAC (Mainsite) | 2023-10-21 | Y | 1 | 10.00 | 100% | 0% | 0% |
Data
| Missouri | WUSTL | 10 | 0 | 0 | 10 |