The V(D)J recombination enzyme tDt can be used with fluorescently tagged deoxynucleotides in an assay for this process. The presence of 180 base pair “laddering” is specific for this process due to CAD activity at linker regions. A heptameric wheel-shaped complex containing Apaf-1 helps mediate this process by recruiting enzymes to its (*) CARD domains. Increased cytosolic-free calcium ions can promote this process through calcineurin mediated dephosphorylation of BAD. The extrinsic pathway of this process begins when the Fas ligand binds to its receptor. During this process, a proteolytic cascade triggers the release of caspases. This process begins when the mitochondrion releases cytochrome c. For 10 points, name this process of programmed cell death ■END■
ANSWER: apoptosis [prompt on cell death]
<JC, Biology>
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