The protein RLI performs this reaction on a “sandwich” of two molecules during ribosome recycling. Proteins like p97 can be studied by swapping this reaction’s reactant with a PNP-containing analogue. In a rate-limiting step, this reaction triggers the binding of a second GroES (“grow-E-S”) lid to a GroEL (“grow-E-L”) chamber after a protein has been folded inside. This reaction is faster than the rate of G-actin addition to the minus end of F-actin, but slower than the rate of addition to the plus end. The (*) “power stroke” is triggered by the release of a molecule produced by this reaction from the head of myosin during muscle contraction. Using the energy of this reaction, a cellular “pump” exchanges three sodium for two potassium ions. For 10 points, what reaction cleaves a high-energy molecule to produce a free phosphate and ADP? ■END■
ANSWER: ATP hydrolysis [accept ATP dephosphorylation or ATP breakdown prompt on hydrolysis or dephosphorylation or breakdown by asking “of what molecule?”]
<AG>
= Average correct buzz position