Question
The “tandem” form of this technique is useful in pro·te·om·ics, since it filters the analytes both before and after fragmentation. For 10 points each:
[10e] Name this analytical technique that separates compounds by their mass-to-charge ratio.
ANSWER: mass spectrometry [accept tandem mass spectrometry or MS/MS]
[10m] Tandem MS is typically implemented with three of these configurations for guiding and filtering ions. At large distances, the electric field created by one of these configurations falls off with the fourth power of distance.
ANSWER: quadrupole [prompt on q or qQq]
[10h] Suppose 5 peptides undergo tandem MS and are each fragmented once, and 3 a-type ions and 2 c-type ions hit the detector. What are the types of the other 5 ions produced in the fragmentation step?
ANSWER: 3 x-type ions AND 2 z-type ions [prompt on x AND z by asking “how many x and how many z?”] (Peptide fragments are classified as a/b/c or x/y/z depending on where the cut is relative to the carbonyl and amine groups; a fragments are complementary to x, b are complementary to y, and c are complementary to z.)
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Summary
| 2023 BHSU @ Berkeley | 03/18/2023 | Y | 3 | 13.33 | 100% | 33% | 0% |
| 2023 BHSU @ Maryland | 03/11/2023 | Y | 3 | 13.33 | 100% | 33% | 0% |
| 2023 BHSU @ Northwestern | 02/25/2023 | Y | 6 | 20.00 | 100% | 100% | 0% |
| 2023 BHSU Online | 04/15/2023 | Y | 4 | 20.00 | 100% | 75% | 25% |
| 2023 BHSU @ Sheffield | 04/15/2023 | Y | 2 | 20.00 | 100% | 100% | 0% |
| 2023 BHSU @ Waterloo | 04/15/2023 | Y | 3 | 20.00 | 100% | 100% | 0% |
Data
| Maryland B+ | Fan-tastic Negs and Where to Find Them | 10 | 0 | 0 | 10 |
| Maryland A | Glass Onyen: A Knives Out Mystery | 10 | 10 | 0 | 20 |
| Rutgers Diaspora | The Perfection of Wisdom in 8,000 Negs | 10 | 0 | 0 | 10 |