Question
The presence of this modification distinguishes the daughter from the template strand during mismatch repair. For 10 points each:
[10m] Name this epigenetic modification common in repetitive sequences and transposons, which is mapped using bisulfite sequencing.
ANSWER: DNA methylation [or methyl group; accept hemimethylation; reject "histone methylation"]
[10h] Two answers required, in the correct order. Treating DNA with bisulfite before sequencing works by converting this nucleobase into this other nucleobase, while leaving methylated sites alone. Spontaneous deamination converts between these two canonical nucleobases.
ANSWER: cytosine to uracil [or C to U; accept cytidine in place of "cytosine"; accept uridine in place of "uracil"; reject "uracil to cytosine" or "U to C"; reject partial answers]
[10e] High levels of methylation at promoter sequences can also interfere with this process, in which RNA polymerase produces messenger RNA from a DNA template.
ANSWER: transcription [accept transcription initiation or transcription elongation; prompt on gene expression or RNA synthesis]
<Biology>
Summary
| 2023 ACF Winter @ Columbia | 11/11/2023 | Y | 4 | 15.00 | 75% | 50% | 25% |