Industrial production of a form of this enzyme active at low pHs relies on Kluyveromyces fragilis (“cloy-VAIR-oh-MY-seez fra-JILL-iss”). A protocol to detect this enzyme involves alkalinization of lysosomes followed by incubation with C12FDG. A form of this enzyme active at a pH of 6 is the most common biomarker for senescent cells. (10[2])The (10[1])pUC19 (10[1])(“puck-nineteen”) plasmid codes for an N-terminal fragment of this enzyme and is used in a molecular cloning screen (10[1]-5[1])that exploits the association between this enzyme’s alpha and omega subunits. PETG (“pet-G”) inhibits this enzyme, while IPTG binds a (10[1])repressor protein (10[1])to induce the transcription of a polycistronic (10[1])mRNA that encodes this enzyme, (10[3])a permease (10[2])(“PERM-ee-ace”), and a transacetylase (10[1])(“trans-uh-SEE-tull-ace”). The blue/white (10[2])screen and other colorimetric (10[1])assays rely on this enzyme’s (10[1])cleavage of X-gal. For 10 points, the lacZ gene encodes (10[1])what enzyme that generates glucose and an eponymous (10[1])monosaccharide by (10[1])cleaving lactose? ■END■ (0[1])