Question

Labcyte’s Echo devices create and eject these objects using acoustic energy. The McCarroll lab developed a protocol named for these objects that makes beads called STAMPs (“stamps”) that are coated in RNA. These objects are made by vortexing Span-80, Tween-80, and sodium deoxycholate (-5[1])(“dee-oxy-KO-late”) in a technique for high-throughput enzyme evolution called IVC. Reagents are added to these objects by picoinjectors. Flow focusing generates these objects by mixing (-5[1])two streams at a T- (10[1])or Y-shaped (-5[1])junction on a chip. Digital (-5[1])PCR generates trillions (10[1])of these objects, (10[1]-5[1])each of which contains zero or one copy of DNA. (10[1])These objects act as mini-reactors in a common format (10[1])for microfluidics, where they are formed (-5[1])by emulsifying picoliters of water in oil. A Taylor cone (10[1])disperses (-5[1])bioanalytes into these objects during electrospray (10[1])ionization. (10[3]-5[1])For 10 points, surface tension (-5[1])holds together (10[1])what spherical beads of liquids? (10[1])■END■ (10[7]0[2])

ANSWER: liquid droplets [accept droplet microfluidics; or Drop-seq; prompt on emulsions until read; prompt on compartments; prompt on micelles; prompt on electrospray]
<Chemistry>
= Average correct buzz position

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Summary

2023 ACF Nationals04/22/2023Y2286%0%41%120.68

Buzzes

PlayerTeamOpponentBuzz PositionValue
Andrew HannaVanderbilt AChicago A41-5
Nikhil ChellamNorthwestern ACornell B65-5
Grace LiuMIT APenn State A7010
Eric WolfsbergStanford ANYU A72-5
Jason HongBrown AGeorgia Tech B77-5
Noah ChenColumbia BToronto A8010
Paul LeeWUSTL APurdue A8310
Geoffrey ChenCornell AChicago B83-5
Daniel SheinbergYale AColumbia A9310
Vincent DuNorth Carolina AVirginia A10210
Ethan AshbrookMinnesota ARutgers A108-5
Rohan ShelkeUC Berkeley BTexas A11810
Conor ThompsonIowa State AUC Berkeley A119-5
Arya KarthikGeorgia Tech AChicago C12510
June YinWUSTL BOhio State A12610
Kevin FanMcGill ARutgers B12610
Todd MaslykMichigan AFlorida B126-5
Mazin OmerHarvard AHouston A12610
Danny HanPenn AMaryland A131-5
Frederick Rivas-GiorgiYale BIndiana A13310
Daniel YangChicago BCornell A13810
Ashish SubramanianDuke AIllinois A13910
S. A. ShenoyGeorgia Tech BBrown A1390
Adam FineChicago AVanderbilt A13910
Dan NiCornell BNorthwestern A13910
Jeya IyaduraiFlorida BMichigan A13910
Shahar SchwartzUC Berkeley AIowa State A13910
Isaac MammelMaryland APenn A1390
Halle FriedmanNYU AStanford A13910
Kevin ParkClaremont AMinnesota B13910