GFP - 2025 ACF Fall - Buzzpoints App

Question

Bacteria transformed with the pGLO (“p-glow”) plasmid can express this protein only when arabinose is present. For 10 points each:

[10h] Name this protein, whose discovery won Tsien, Shimomura, and Chalfie the 2008 Nobel Prize in Chemistry. This protein, which was first isolated from Aequorea victoria, has an emission peak at roughly 509 nanometers.

ANSWER: GFP [or green fluorescent protein]

[10m] The pGLO plasmid’s bla gene codes for an enzyme that hydrolyzes the beta-lactam type of these compounds. Pen-Strep is a cocktail of these compounds widely used in cell culture to prevent contamination.

ANSWER: antibiotics [accept antimicrobials; prompt on penicillin or streptomycin or penicillin-streptomycin by asking “what more general type of compound is that?”]

[10e] Like most circular plasmids, the pGLO plasmid initiates this process at its ori (“OR-ee”) site. This semiconservative process uses polymerases to duplicate strands of DNA.

ANSWER: DNA replication [accept origin of replication]

<Editors, Biology>

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Summary


Northern California	Main	Y	4	20.00	75%	100%	25%
Southern California	Main	Y	6	15.00	83%	50%	17%
Eastern Canada (1)	Main	Y	4	15.00	100%	50%	0%
Eastern Canada (2)	Main	Y	7	15.71	57%	57%	43%
Florida	Main	Y	1	10.00	100%	0%	0%
Great Lakes	Main	Y	11	18.18	73%	64%	46%
Lower Mid-Atlantic	Main	Y	7	18.57	100%	57%	29%
Upper Mid-Atlantic	Main	Y	9	13.33	89%	33%	11%
Upper Mid-Atlantic	Main	Y	1	10.00	0%	0%	100%
Midwest	Main	Y	9	13.33	44%	56%	33%
North	Main	Y	4	10.00	50%	50%	0%
Northeast	Main	Y	11	18.18	91%	73%	18%
Pacific	Main	Y	8	12.50	63%	38%	25%
South Central	Main	Y	6	10.00	50%	50%	0%
Southeast	Main	Y	10	13.00	60%	50%	20%
UK (North)	UK	Y	4	17.50	75%	75%	25%
UK (South)	UK	Y	5	6.00	20%	40%	0%
Upstate NY	Main	Y	4	7.50	50%	25%	0%

Data


Berkeley Mighty Miner	Berkeley Archer Queen	0	10	10	20
Berkeley Boss Bandit	Stanford B	0	10	10	20
Berkeley Skeleton King	Berkeley Little Prince	10	10	10	30
UCSC	Stanford A	0	10	0	10
UC Irvine	Claremont C	0	10	10	20
UC Santa Barbara	UCLA C	0	0	10	10
UCLA B	Claremont B	0	0	10	10
Claremont A	UCLA D	0	10	10	20
UCSD B	UCSD A	10	10	10	30
McGill C	McGill A	0	10	10	20
Ottawa D	McGill B	0	0	10	10
UCLA E	USC A	0	0	0	0
Carleton A	McGill E	0	10	10	20
Ottawa A	Ottawa B	0	0	10	10
Toronto E	Guelph A	0	0	0	0
Toronto F	McMaster C	10	10	10	30
Toronto A	Waterloo A	0	10	0	10
Toronto B	Toronto C	10	10	10	30
Toronto D	McMaster A	10	0	10	20
Waterloo B	Toronto G	0	0	0	0
Waterloo C	McMaster B	0	10	10	20
Florida A	UCF A	0	0	10	10
CWRU B	CWRU A	10	10	10	30
Kentucky	Pittsburgh	0	10	10	20
Ohio State A	Louisville B	10	10	10	30
Louisville C	Michigan State D	0	0	0	0
Michigan B	Michigan A	10	10	10	30
Michigan C	CWRU D	0	0	0	0
CWRU C	Michigan D	10	0	0	10
Michigan State A	Louisville A	0	10	10	20
Ohio State B	Michigan State B	10	10	10	30
Ohio State C	Louisville D	0	10	10	20
Ohio State D	Michigan State C	0	0	10	10
Virginia Tech A	UNC A	10	10	10	30
NCSU A	William & Mary A	0	10	10	20
NCSU B	Liberty B	0	0	10	10
UNC C	UNC B	0	0	10	10
Virginia Tech B	Virginia A	0	10	10	20
UNC D	Virginia Tech C	10	10	10	30
William & Mary B	JMU A	0	0	10	10
George Mason C	Columbia B	0	0	0	0
Johns Hopkins	George Mason B	10	10	10	30
Lehigh B	NYU B	0	0	10	10
Rutgers A	Maryland A	0	0	10	10
NYU A	Maryland C	0	10	10	20
Rowan	Vassar B	0	0	10	10
Rutgers C	NYU C	0	0	10	10
Penn	Rutgers D	0	10	10	20
Stony Brook	Vassar A	0	0	10	10
Chicago A	WashU Grizzlers	10	0	0	10
Purdue A	Chicago B	10	10	10	30
Iowa B	Illinois Champaign	0	0	0	0
Illinois Orange	Indiana B	0	10	0	10
Illinois Canada	Purdue B	0	0	10	10
Iowa A	Purdue C	10	10	10	30
Rose-Hulman A	Purdue D	0	0	10	10
Indiana A	WashU Grolars	0	10	0	10
Illinois Blue	William Woods University A	0	10	0	10
Minnesota	Wisconsin B	0	0	0	0
St. Thomas	Macalester	0	0	0	0
Wisconsin A	Iowa State	0	10	10	20
Wisconsin C	Michigan Tech	0	10	10	20
Boston University B	Amherst B	0	0	10	10
Brandeis Mozart	Boston College A	0	0	10	10
Brown A	Dartmouth A	0	10	10	20
MIT A	Brown B	10	10	10	30
Harvard	Brandeis Beethoven	10	10	10	30
Tufts A	Amherst A	0	10	0	10
Vermont A	Tufts B	0	10	10	20
UConn A	Boston University A	0	10	10	20
Vermont B	Williams A	0	0	10	10
Dartmouth B	Williams B	0	10	10	20
Williams C	Dartmouth C	0	10	10	20
Alberta A	Washington Atlantic	10	0	10	20
Alberta B	Washington Chum	0	0	0	0
Arizona State A	Washington Sockeye	0	0	10	10
Texas	British Columbia B	0	10	10	20
Washington State	British Columbia C	0	10	0	10
Washington Steelhead	Washington Chinook	0	10	10	20
Washington Coho	British Columbia A	0	0	0	0
Washington Pink	Washington Masu	10	0	10	20
Tulsa B	Central Oklahoma B	0	10	0	10
Murray State College B	Murray State College A	0	0	0	0
Nebraska B	Nebraska A	0	0	0	0
Oklahoma	Nebraska C	0	10	10	20
Harding	Rose State	0	0	10	10
Central Oklahoma A	Tulsa A	0	10	10	20
Vanderbilt A	Alabama A	0	0	10	10
Auburn A	Tennessee Tech	0	10	0	10
Clemson A	Chipola College	10	10	10	30
Montevallo	Clemson B	0	0	0	0
Emory A	Georgia A	0	10	0	10
Georgia Tech B	Auburn B	10	10	10	30
Georgia Tech A	Georgia Tech C	0	0	10	10
Georgia Tech F	Georgia Tech E	0	10	10	20
Tennessee A	South Carolina A	0	0	10	10
Georgia Tech D	Tennessee B	0	0	0	0
George Mason A	Maryland B	10	0	0	10
Sheffield A	Edinburgh A	10	10	10	30
Durham B	Edinburgh B	0	0	10	10
Durham A	Edinburgh C	0	10	10	20
Sheffield B	Durham C	0	10	0	10
Cornell A	Cornell D	0	10	10	20
Cambridge A	Southampton	0	10	0	10
Cambridge D	Imperial A	0	10	0	10
Birmingham	Imperial B	0	0	0	0
Warwick	LSE	0	0	10	10
Oxford	Imperial D	0	0	0	0
Cornell C	Binghamton B	0	0	10	10
RIT A	ESF	0	0	0	0
Binghamton A	RIT B	0	0	0	0