The yeast protein TP-DNAP1 is used in a high-throughput platform for this technique called OrthoRep. The induced expression of protein III (“three”) is monitored in a form of this technique that continuously pumps E. coli into a “lagoon” infected with M13. A form of this technique (15[1])was first used to develop a subtilisin E variant that is stable in DMF. “Continuous” forms of this technique include a “phage-assisted” one developed by (15[1])David (15[1])Liu’s lab, called (*) PACE. (10[1])Aptamers (10[2])are synthesized and enriched for high binding affinity with an in vitro form of this process called (10[1])SELEX (“see-lecks”). Rational design is contrasted with a technique named for this process (10[1])that was pioneered by Frances Arnold and is used to discover novel enzymes. For 10 points, what process underpins a “directed” form of protein (10[1])engineering that uses cycles of library generation and mutagenesis to mimic natural selection? ■END■ (0[4])