This assay, which is often followed by qPCR, is the subject of the ENCODE Significance Tool. For 10 points each:
[10m] Name this assay in which magnetic beads conjugated to proteins A and G are used to pull down antibodies bound to proteins of interest. CUT&RUN has largely superseded this technique.
ANSWER: ChIP [or chromatin immunoprecipitation; accept ChIP-Seq or ChIP-sequencing; prompt on IP or immunoprecipitation]
[10e] In the first step of ChIP, relevant proteins are cross-linked to DNA using the “para” form of this compound. This toxic molecule is produced by oxidizing methanol.
ANSWER: formaldehyde [or paraformaldehyde; or formalin; or methanal; accept CH2O; prompt on PFA]
[10h] Native ChIP skips cross-linking and instead shears the chromatin using a nuclease from this genus. The high GC content of this genus’s luteus species is an early example of an evolving codon system.
ANSWER: Micrococcus [accept Micrococcus luteus; accept micrococcal nuclease; prompt on MNase]
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