In one technique, the pIII (“P-three”) protein of one of these organisms allows it to attach to the F pilus of a pathogen. For 10 points each:
[10m] Name these organisms used in a namesake “assisted continuous evolution” technique. Libraries from these organisms undergo multiple rounds of affinity selection followed by amplification in a namesake “display.”
ANSWER: bacteriophages [accept phage display or phage-assisted continuous evolution; accept lambda phage or M13 phage; prompt on viruses]
[10e] The M13 phage used in phage-assisted continuous evolution replicates by infecting this pathogen. Hershey and Chase used a T2 phage to infect this rod-shaped Gram-negative bacteria that can cause food poisoning.
ANSWER: E. coli [or Escherichia coli]
[10h] The M13 phage is also used in Kunkel’s method of performing this technique using mutated E. coli strains. Either this technique or error-prone PCR is used to generate libraries in alanine scanning.
ANSWER: site-directed mutagenesis [or site-specific mutagenesis or oligonucleotide-directed mutagenesis; prompt on SDM or mutagenesis or point mutation or mutation scanning or deep mutation scanning]
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