Question

A class of enzymes that perform this process with a catalytic serine residue is exemplified by Bxb1 and PhiC31 (10[1])and is used to deliver large cargo. An enzyme that performs this process is delivered alongside various fluorescent proteins to visualize the connectome in Brainbow imaging. This process can be encouraged by knocking out Ku·70 and Ku·80 (10[1])to inhibit a different process from competing with this one during CRISPR knock-in experiments. This process is (10[1])performed by a non-chaperone (10[1])enzyme that, when attached to an estrogen receptor, creates an inducible (10[1])gene expression system controlled by tamoxifen. This process is localized to LoxP sites (10[2])in a site-specific (10[1])variant (10[1])of this process (10[2])driven by (-5[1])the enzyme Cre (-5[1])(“cree”). For 10 points, name this process in which two homologous strands of DNA exchange sequences (10[2])with (10[1])each other. (10[1])■END■ (10[3]0[3])

ANSWER: homologous recombination [accept HR; accept recombinases or Cre recombinase; accept large serine recombinases; accept genetic recombination; accept recombineering; accept crossing over; accept homology-directed repair; prompt on insertion or integration; prompt on DNA repair or double-strand break repair or DSB repair]
<Biology>
= Average correct buzz position

Summary

2025 ACF Nationals04/19/2025Y2090%0%10%101.89

Buzzes

PlayerTeamOpponentBuzz PositionValue
Jason ZhangToronto CRIT1810
Sharath NarayanNorthwesternStanford5510
Bryan UgazArizona StateWaterloo B7210
Raymond ChenToronto BWinona State7610
Collin NadarajahWUSTL BColumbia A8710
David BassJohns HopkinsGeorgia State10010
Annabelle YangWUSTL AMIT10010
Andrew WangIllinois AVirginia10310
Richard NiuCornell AOttawa10410
Tony ChenToronto AGeorgia Tech10710
Ryan RosenbergNYUPenn State10710
Tiffany ZhouFloridaIllinois B109-5
Alan XieYaleCornell B112-5
Shiva TegullaUCFOhio State12710
Joel MilesMinnesotaMichigan12710
Andy HuffLSEVanderbilt12810
Soren HeydariNorth Carolina BTexas13010
Yash MandaviaIllinois BFlorida1310
Alex AkridgeIndianaIowa State13110
Liam KusalikWaterloo ANorth Carolina A13110
Andy YuMarylandHarvard1310
Michael KearneyHarvardMaryland1310
Julian BushlowCornell BYale13110