Although unrelated to serum albumin, this process can be detected by visualizing the uptake of Evans blue. Despite the high throughput of protoplast assays, the increased risk of this process makes those assays notoriously difficult to perform consistently. This process is induced by the second of three buffers added at the start of a miniprep (10[1])protocol, (10[1])often denoted (10[1])P2. (10[1]-5[1])An enzyme named for performing this process (10[1])had its crystal structure derived by David Phillips after it was isolated from hen egg whites. (-5[1])Cells undergo this (-5[1])process after being disturbed by pulses of sound (10[1])waves (10[2])in sonication. (10[3])Protein (10[1])purifications (10[1])induce this process by using detergents like (10[1])SDS to (-5[1])lower (-5[1])the surface tension of the cell membrane. (10[1])For 10 points, (-5[2])moving a cell without a cell wall into a hypotonic environment will cause what process in which (-5[1])a cell bursts? ■END■ (10[8])