Question

The assumption that this ratio is consistent often fails due in part to the variable activity of two degradation pathways that occur before and after the action of eIFs. For 10 points each:
[10h] Describe this ratio whose variability can be controlled for by comparing real-time cDNA readouts to those of a quantitative Western blot.
ANSWER: transcription to translation ratio [accept ratio of mRNA to protein; accept translation to transcription or protein to mRNA]
[10e] The correlation between mRNA and protein production often breaks down at these organelles, whose binding sites are encoded by mRNAs in the five-prime untranslated region. These organelles perform translation.
ANSWER: ribosomes
[10m] A variant of qPCR that uses this enzyme is a cheap and transcript-specific way to quantify mRNA levels without resorting to an RNA sequencing or Northern blot run. Variants of PCR that use this enzyme generate cDNA.
ANSWER: reverse transcriptase [accept RT]
<Biology>

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Summary

2025 ACF Nationals04/19/2025Y1619.38100%88%6%

Data

UC Berkeley BArizona State0101020
British ColumbiaLSE0101020
RutgersChicago A0101020
Cornell ATexas0101020
FloridaNYU010010
YaleGeorgia State0101020
IndianaIllinois A0101020
Virginia TechIowa State0101020
MITToronto C0101020
North Carolina AColumbia A0101020
StanfordToronto A0101020
Toronto BHarvard0101020
UC Berkeley AWaterloo A10101030
UCFCornell B0101020
WUSTL ANorthwestern0101020
Penn StateWUSTL B010010