One form of this technique uses tagmentation to fragment samples and add adapters simultaneously (10[1])for library preparation. (10[2])Solexa pioneered a form of this technique in which a sample in a flow cell (10[1])is subjected to bridge amplification. (-5[2])Samples (10[1]-5[1])are treated with (-5[1])bisulfite in one form (10[2])of this (-5[1])technique (10[2])to determine methylation patterns. (10[1])Another (-5[1])form of this technique (10[2]-5[1])uses capillary (10[1])electrophoresis (-5[1])to migrate samples (-5[2])through a fluorescence (-5[4])detector (10[1]-5[4])to generate (10[1]-5[1])a chromatogram. (10[2]-5[5])That (-5[1])form (10[1]-5[1])of this technique (-5[1])relies (10[1])on chain (10[1]-5[1])termination (-5[3])by randomly (10[1]-5[1])incorporated (10[2]-5[3])ddNTPs (10[4]-5[3])and is named for (-5[1])Sanger. (10[1])For (10[1])10 (10[1])points, (10[5]-5[1])name (10[1])this technique (10[4])used (10[1])to determine the order (10[1])of nucleotides (10[3]-5[2])in (10[1])DNA. (10[3])■END■ (10[43]0[26])