This technique is the most common use of 3-hydroxypicolinic acid to detect nucleotides. It’s not UV-Vis spec, but digital asymmetric wave isolation for this technique was developed by the Shimadzu company, which sells “mini” instruments for it. A breakthrough in this technique was the use of a solution of 30-nanometer cobalt particles in glycerol. The analyte in this technique can be described by the “gas-phase protonation” or “Lucky Survivor” models. It’s not SPPS, but this technique uses trifluoroacetic acid as a counterion source to a structure made of DHB, alpha-CHCA, or (*) sinapinic acid. Hillenkamp and Karas developed this “soft” fragmentation technique, which is often combined with a time-of-flight analyzer. For 10 points, name this mass spectrometry technique in which large samples are ionized within a crystal structure that absorbs a beam of coherent light. ■END■
ANSWER: MALDI [or matrix-assisted laser desorption/ionization; accept MALDI-time-of-flight or MALDI-TOF; accept MALDImini; prompt on mass spectrometry or MS until read; prompt on time-of-flight mass spectrometry or TOF-MS until “time-of-flight” is read; prompt on soft laser desorption or SDL; prompt on laser desorption/ionization; prompt on soft ionization until “ionized” is read; prompt on digital ion trap or DIT] (The 30-nanometer cobalt clue refers to Koichi Tanaka and SDL, which improved on the work of Hillenkamp and Karas.)
<KP, Chemistry>
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