Classically, a caged molecule has a protecting group removed by this stimulus. For 10 points each:
[10e] Name this stimulus that can excite electrons enough to cleave bonds.
ANSWER: light [accept visible light, ultraviolet light, UV light, infrared light, or IR; prompt on electromagnetic radiation]
[10m] Photoclick decaging is a common means of forming prodrugs that adhere to this principle. This approach was developed in the Bertozzi lab to study the glycocalyx and consists of reactions whose components are effectively absent from living systems and are thus unaffected by enzymes.
ANSWER: bioorthogonal chemistry [or bioorthogonality; prompt on orthogonal chemistry or orthogonality]
[10h] The biosynthesis of this probe molecule involves a click reaction between a nitrile and the thiol of cysteine. After being adenylated, this molecule is oxidized to form a dioxetane ring that quickly decomposes.
ANSWER: luciferin [accept firefly luciferin or beetle luciferin]
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