Purified proteins are often stored in solutions containing Tris (“triss”), an inert example of this type of base. For 10 points each:
[10e] Name this type of acids and bases that include ammonia and acetic acid. Buffers contain this type of acid or base that only partly ionizes.
ANSWER: weak acids or bases [or word forms like weakly ionizing]
[10m] For long-term storage, protein buffers also usually include this three-carbon alcohol as a cryoprotectant. Three fatty acids are attached to this backbone molecule in a common type of fat.
ANSWER: glycerol [or glycerine; or 1,2,3-propanetriol]
[10h] Salts of this inorganic ion are so toxic that they are often added to protein stocks as antimicrobial agents. Organic compounds containing this ion are much safer; they click onto alkynes in the key reaction of bioorthogonal chemistry.
ANSWER: azides [or sodium azide; or N3−]
<Jonathen Settle, Science - Chemistry> ~19207~ <Editor: Adam Silverman>