Tissue imaging with minimal sample damage can be achieved using this technique’s “static” mode, which most often employs a low-power caesium or gallium beam. For 10 points each:
[10h] Name this technique whose static mode provides spatially resolved monolayer compositional information, acapability its small-molecule counterpart lacks.
ANSWER: secondary ion mass spectrometry [or SIMS; accept TOF-SIMS; prompt on mass spectrometry or MS]
[10e] Dynamic secondary ion mass spectrometry is a powerful technique for profiling with respect to this parameter. Argon sputtering in XPS allows for compositional measurement with respect to this physical parameter.
ANSWER: depth [or height or z-axis; accept depth profiling]
[10m] To avoid contamination of the SIMS vacuum environment, biological samples are often prepared in this manner. Electron microscopy of biological samples often uses this technique to avoid sample damage and preserve native structures.
ANSWER: freezing [or cryopreservation]
<Vittal Bhat, Chemistry>