Question
Answer the following about the type II (“2”) secretion system. For 10 points each:
[10e] Type II (“2”) secretion is a GSP-mediated process found in bacteria that score a “negative” result in this staining technique that employs iodine as a mordant and crystal violet as an initial dye.
ANSWER: Gram stain [or Gram’s stain or Gram’s method; accept Gram-negative bacteria]
[10m] Exoproteins destined to leave a Gram-negative bacterial cell via type II secretion must first be transported into this location, the space between its inner and outer membranes.
ANSWER: periplasm [or periplasmic space]
[10h] The gram-negative bacterium P. carotovorum uses the type II secretion system to export virulence factors that break down these molecules. These biopolymers are primarily composed of galacturonic acid monomers.
ANSWER: pectins [accept pectinases; accept Pectobacterium carotovorum; accept Pectobacteriaceae]
<Kane Nguyen, Biology>
Summary
| 2024 Penn Bowl CWRU | 11/02/2024 | Y | 1 | 10.00 | 100% | 0% | 0% |
| 2024 Penn Bowl UNC | 10/26/2024 | Y | 2 | 15.00 | 100% | 50% | 0% |