Question
Many forms of this technique employ Sephadex beads to purify proteins and oligonucleotides. For 10 points each:
[10e] Name this technique used to separate compounds based on their ability to elute through a stationary phase. This technique has size-exchange and thin-layer varieties.
ANSWER: chromatography [accept column chromatography; accept ion-exchange chromatography; accept affinity chromatography; accept high-performance liquid chromatography; accept size-exchange chromatography or molecular sieve chromatography or gel-filtration chromatography; accept thin-layer chromatography; prompt on IEC or HPLC or SEC or TLC]
[10m] In one form of chromatography, Sephadex beads coated with streptavidin are used to purify peptides conjugated to this molecule. This vitamin is available at lower levels in people who regularly eat raw egg whites.
ANSWER: biotin [or vitamin B7 or vitamin H; prompt on vitamin B]
[10h] In the 1970s, Jan Sjövall developed hydroxyalkoxy-modified Sephadex beads, which he used to purify these compounds from bodily fluids. In 1963, Torgov synthesized one of these compounds using an AB to ABD to ABCD approach.
ANSWER: steroids [or sterols; accept sex steroids; accept corticosteroids; accept cholesterols; anti-prompt on estrogens; anti-prompt on estrones; anti-prompt on androgens; anti-prompt on progestins or progestogens or progestagens or gestagens; anti-prompt on corticoids; anti-prompt on sex hormones]
<Kane Nguyen, Chemistry>
Summary
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